• The authentic PPase was purified from B subtilis as describe

  2019-07-26

  

  The authentic PPase was purified from B. subtilis as described previously [21], whereas the recombinant enzyme was purified to homogeneity from E. coli by a simplified procedure including only phenyl-Sepharose CL-4B column chromatography and DEAE-HPLC [21]. About 26 mg of pure enzyme was obtained fr

• br Fragment based drug discovery FBDD is a powerful

  2019-07-26

  

  Fragment-based drug discovery (FBDD) is a powerful method to discover drug leads and has been widely adopted in both academia and industry. FBDD can be used to explore chemical all trans retinoic acid where space with libraries which are smaller in size, producing drug leads with high ligand-bindi

• In order to gain further insights into the role of

  2019-07-26

  

  In order to gain further insights into the role of peptidases in B. xylophilus, four cysteine proteases highly secreted by B. xylophilus (Cardoso et al., 2016) were selected four further characterisation. Materials and methods Results and discussion Structural prediction and analysis The i

• Ketorolac tromethamine salt for The inhibition of FAS by C

  2019-07-26

  

  The inhibition of FAS by C75 produces an accumulation of malonyl-CoA which is difficult to reconcile with the activation of CPT1 reported by others [2], [16], [17], [18]. To unravel this paradox the mechanism of action of C75 needs to be examined. We recently demonstrated that C75 is converted in vi

• br Material and methods br

  2019-07-26

  

  Material and methods Results Discussion Conclusions To conclude, although CPT2 and CPT1 must have co-evolved to establish the carnitine shuttling, these enzymes are the most distantly related carnitine transferases, split early in evolution, during which a switch occurred in their locati

• ClC is not only a chloride

  2019-07-26

  

  ClC-3 is not only a chloride ion channel but also a regulatory protein involved in the regulation of multiple signaling pathways. The PI3K/AKT/mTOR signaling pathway plays a crucial role in regulating cell growth, migration, invasion, and distant metastasis [13,14]. Although abnormal PI3K/AKT/mTOR a

• Materials and methods Construction of the Chk

  2019-07-26

  

  Materials and methods Construction of the Chk-YB-1b-Neo vector. Approximately 107 plaques from a chicken genomic library established in EMBL3 vector (Clontech Palo Alto, CA) was screened and one clone containing a 12kb insert was selected. Digestion of this clone with SalI and EcoRI restriction enz

• Although in vitro and in vivo immunosuppressive

  2019-07-26

  

  Although, in vitro and in vivo immunosuppressive effects of female steroids have been described in fish (Yamaguchi et al., 2001, Law et al., 2001), their mode of action was not evaluated in detail. Consequently, it is of considerable interest to investigate the intracellular pathways that regulate t

• The nitrophenols b d were prepared as

  2019-07-26

  

  The nitrophenols 34b–d were prepared as described in Scheme 3b. Nitration of phenols 39b–d with 1equiv of sodium nitrate in the presence of hydrochloric Manumycin A gave an inseparable mixture of 34b–d and 40b–d (1:1). This synthetic problem was successfully avoided as described below. Nitration of

• Our results from neutropenic mice

  2019-07-26

  

  Our results from neutropenic mice infected with both fungal strains revealed that the inflammatory process was more severe 72hours post-infection (Fig. 4). Angioinvasion was mainly observed 48hours after infection for both strains. Transformation of A. fumigatus conidia into hyphae in immunocompeten

• Through an unknown mechanism RING Ubox

  2019-07-26

  

  Through an unknown mechanism, RING/Ubox-type E3 dimers and the N-terminal tail have been shown to function as a modulator of full E3 ubiquitin ligase activity [27]. LRSAM1 promotes a significant enhancement in the formation of the high-molecular-weight products or E3 activity when the RING domain is

• It is also critical that APC CCDC is inhibited by

  2019-07-25

  

  It is also critical that APC/CCDC20 is inhibited by MCC during the spindle assembly checkpoint, prior to correct chromosome alignment on the mitotic spindle (Box 1, reviewed in 13, 34). As proposed in [13] and demonstrated in [82], human MCC is a heterotetrameric complex consisting of its own molecu

• br Introduction Identification and correct storage of

  2019-07-25

  

  Introduction Identification and correct storage of seminal stains are crucial for the investigation of sexual assault cases. Actually, undetected ‘cold’ cases involving sexual assault can be solved decades after investigations analysing DNA from stored evidences [1]. Recently, STR analysis and th

• br Materials and Methods br Results

  2019-07-25

  

  Materials and Methods Results Discussion HSCs have been identified as major collagen-producing prostaglandin synthase in the injured liver (1), and activation of HSCs has become the central link for liver fibrosis (2). The factors that regulate the biological behavior of HSCs include solubl

• br Chagas disease and HAT drug

  2019-07-25

  

  Chagas disease and HAT drug R&D Drug R&D for Chagas disease and HAT has historically relied on serendipitous findings achieved using low-technology approaches. The absence of biotechnology tools associated and the very limited understanding of the biomolecular processes of the parasites are among

16513 records 1048/1101 page Previous Next First page 上5页 10461047104810491050 下5页 Last page